Jeong Chan Park, Do Hyeon Kim, Chang Sup Kim, and Jeong Hyun Seo
Jeong Chan Park†
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea
Do Hyeon Kim†, Jeong Hyun Seo*
School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea
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Chang Sup Kim
School of Chemistry and Biochemistry, Yeungnam University, Gyeongsan 38541, Korea
†These authors contributed equally to this work.
Received: 7 November 2017 / Revised: 2 January 2018 / Accepted: 18 January 2018
© The Korean Society for Biotechnology and Bioengineering and Springer 2018
We examined the performance of methyltrimethoxysilane (MTMS), a precursor of silicic acid, in the process of biosilicification induced by the R5 peptide from Cylindrotheca fusiformis. Recombinant GFP-R5 fusion protein was produced by Escherichia coli cultured at 25°C as a soluble and functional formation, but not at 37°C. MTMS-based biosilica deposits had a larger average diameter compared to tetraethyl orthosilicate (TEOS)-based deposits. Reducing phosphate concentration in the buffer system led to a decrease in the size of MTMS-based biosilica. These results provide insight into the surface modification of biosilica, and control of biosilica particle size, when using hydrophobic precursors such as MTMS.
methyltrimethoxysilane, biosilica, hydrophobic silane, GFP-R5 fusion system