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R5 Peptide-based Biosilicification Using Methyltrimethoxysilane

Jeong Chan Park, Do Hyeon Kim, Chang Sup Kim, and Jeong Hyun Seo


Jeong Chan Park†
Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Korea
Do Hyeon Kim†, Jeong Hyun Seo*
School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Korea
Tel: +82-53-810-2525; Fax: +82-53-810-4631; E-mail: jhseo78@yu.ac.kr
Chang Sup Kim
School of Chemistry and Biochemistry, Yeungnam University, Gyeongsan 38541, Korea
†These authors contributed equally to this work.

 

Received: 7 November 2017 / Revised: 2 January 2018 / Accepted: 18 January 2018
© The Korean Society for Biotechnology and Bioengineering and Springer 2018


Abstract

We examined the performance of methyltrimethoxysilane (MTMS), a precursor of silicic acid, in the process of biosilicification induced by the R5 peptide from Cylindrotheca fusiformis. Recombinant GFP-R5 fusion protein was produced by Escherichia coli cultured at 25°C as a soluble and functional formation, but not at 37°C. MTMS-based biosilica deposits had a larger average diameter compared to tetraethyl orthosilicate (TEOS)-based deposits. Reducing phosphate concentration in the buffer system led to a decrease in the size of MTMS-based biosilica. These results provide insight into the surface modification of biosilica, and control of biosilica particle size, when using hydrophobic precursors such as MTMS.


Keywords

methyltrimethoxysilane, biosilica, hydrophobic silane, GFP-R5 fusion system

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